| Alternative title | OMIC-1 : Matériau de référence pour la glycoprotéine spiculaire du variant Omicron BA.4/5 du SRAS-CoV-2 |
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| DOI | Resolve DOI: https://doi.org/10.4224/crm.2023.omic-1 |
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| Author | Search for: Stocks, Bradley1ORCID identifier: https://orcid.org/0000-0002-7265-9344; Search for: Thibeault, Marie-Pier1 |
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| Affiliation | - National Research Council of Canada
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| Format | 3D, Material |
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| Material type | Calibration solution |
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| Subject | Canadian reference material; OMIC-1; SARS-CoV-2 spike glycoprotein |
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| Abstract | OMIC-1 is a reference material (RM) designed for the development of SARS-CoV-2 BA.4/5 spike glycoprotein detection methods, as well as an antigen source for use in SARS-CoV-2 immunological assays. Reference values for OMIC-1 are based on combined results from two orthogonal methods from data generated at the National Research Council of Canada (NRC). A unit of OMIC-1 contains 0.2 mL of SARS-CoV-2 BA.4/5 spike glycoprotein in Dulbecco's phosphate-buffered saline (DPBS) with 10 mM HEPES sodium salt. |
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| Analytes | | Analyte | Quantity | Value | Expanded uncertainty | Unit | Type |
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| SARS-CoV-2 BA.4/5 spike protein | molar concentration | 5.3 | 0.6 | µmol/L | reference | | SARS-CoV-2 BA.4/5 spike protein | mass fraction | 0.75 | 0.08 | mg/g | reference | | SARS-CoV-2 BA.4/5 spike protein | mass concentration | 0.76 | 0.08 | mg/mL | reference |
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| Period of validity | - Valid from 2023-03-01 until 2032-11-30
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| Storage conditions | |
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| Publication date | 2023-03-01 |
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| Publisher | National Research Council of Canada |
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| Copyright statement | - © 2023
National Research Council of Canada
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| Access condition | |
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| References | View items (5)- Stocks, Bradley B., Marie-Pier Thibeault, Denis L’Abbé, Muhammad Umer, Yali Liu, Matthew Stuible, Yves Durocher, and Jeremy E. Melanson. ‘Characterization of Biotinylated Human ACE2 and SARS-CoV-2 Omicron BA.4/5 Spike Protein Reference Materials’. Analytical and Bioanalytical Chemistry 416, no. 22 (28 June 2024): 4861–72. https://doi.org/10.1007/s00216-024-05413-7.
- Stuible, Matthew, Christian Gervais, Simon Lord-Dufour, Sylvie Perret, Denis L’Abbé, Joseph Schrag, Gilles St-Laurent, and Yves Durocher. ‘Rapid, High-Yield Production of Full-Length SARS-CoV-2 Spike Ectodomain by Transient Gene Expression in CHO Cells’. Journal of Biotechnology 326 (January 2021): 21–27. https://doi.org/10.1016/j.jbiotec.2020.12.005.
- Isho, Baweleta, Kento T. Abe, Michelle Zuo, Alainna J. Jamal, Bhavisha Rathod, Jenny H. Wang, Zhijie Li, et al. ‘Persistence of Serum and Saliva Antibody Responses to SARS-CoV-2 Spike Antigens in COVID-19 Patients’. Science Immunology 5, no. 52 (8 October 2020). https://doi.org/10.1126/sciimmunol.abe5511.
- Veen, Adriaan M. H. van der. ‘Bayesian Analysis of Homogeneity Studies in the Production of Reference Materials’. Accreditation and Quality Assurance 22, no. 6 (2 November 2017): 307–19. https://doi.org/10.1007/s00769-017-1292-6.
- ‘Evaluation of Measurement Data — Guide to the Expression of Uncertainty in Measurement’, 2008. https://doi.org/10.59161/jcgm100-2008e.
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| Export citation | Export as RIS |
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| Collection | Canadian Reference Materials and Methods |
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| Record identifier | 5bff7c51-00c7-42ee-ba32-8b20d884b824 |
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| Record created | 2023-03-29 |
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| Record modified | 2025-06-21 |
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