| Alternative title | OMIC-1 : Matériau de référence de la glycoprotéine spiculaire du variant Omicron BA.4/5 du SRAS-CoV-2 |
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| DOI | Resolve DOI: https://doi.org/10.4224/crm.2023.omic-1 |
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| Author | Search for: Stocks, Bradley1ORCID identifier: https://orcid.org/0000-0002-7265-9344; Search for: Thibeault, Marie-Pier1 |
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| Affiliation | - National Research Council Canada
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| Format | 3D, Material |
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| Subject | Canadian reference material; OMIC-1; SARS-CoV-2 spike glycoprotein |
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| Abstract | OMIC-1 is a reference material (RM) designed for the development of SARS-CoV-2 BA.4/5 spike glycoprotein detection methods, as well as an antigen source for use in SARS-CoV-2 immunological assays. Reference values for the molar concentration, mass fraction, and mass concentration of spike glycoprotein in OMIC-1 have been established. Values are provided for the spike protein amino acid sequence region that ignores the glycan, and also the full spike glycoprotein where the mass fraction and mass concentration values include an estimate of the molar mass of the attached glycans. Information values for the size heterogeneity of the spike glycoprotein in OMIC-1 are provided. A unit of OMIC-1 contains 0.2 mL of SARS-CoV-2 BA.4/5 spike glycoprotein in Dulbecco's phosphate buffered saline (DPBS) with 10 mM HEPES sodium salt. |
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| Analytes | | Analyte | Quantity | Value | Expanded uncertainty | Unit | Type |
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| SARS-CoV-2 BA.4/5 spike protein | molar concentration | 5.4 | 0.5 | µmol/L | reference | | SARS-CoV-2 BA.4/5 spike protein | mass fraction | 0.75 | 0.07 | mg/g | reference | | SARS-CoV-2 BA.4/5 spike protein | mass concentration | 0.76 | 0.07 | mg/mL | reference | | SARS-CoV-2 BA.4/5 spike glycoprotein | molar concentration | 5.4 | 0.5 | µmol/L | reference | | SARS-CoV-2 BA.4/5 spike glycoprotein | mass fraction | 0.97 | 0.10 | mg/g | reference | | SARS-CoV-2 BA.4/5 spike glycoprotein | mass concentration | 0.97 | 0.10 | mg/mL | reference |
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| Publication date | 2023-03-01 |
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| Publisher | National Research Council of Canada |
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| Copyright statement | - © 2023 National Research Council of Canada
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| Access condition | |
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| References | View items (4)- Stocks, Bradley B., Marie-Pier Thibeault, Joseph D. Schrag, and Jeremy E. Melanson. ‘Characterization of a SARS-CoV-2 Spike Protein Reference Material’. Analytical and Bioanalytical Chemistry 414, no. 12 (9 March 2022): 3561–69. https://doi.org/10.1007/s00216-022-04000-y.
- Guide to the expression of uncertainty in measurement (GUM) (1 September 2008). https://www.bipm.org/en/publications/guides/gum.html.
- Stuible, Matthew, Christian Gervais, Simon Lord-Dufour, Sylvie Perret, Denis L’Abbé, Joseph Schrag, Gilles St-Laurent, and Yves Durocher. ‘Rapid, High-Yield Production of Full-Length SARS-CoV-2 Spike Ectodomain by Transient Gene Expression in CHO Cells’. Journal of Biotechnology 326 (January 2021): 21–27. https://doi.org/10.1016/j.jbiotec.2020.12.005.
- Veen, Adriaan M. H. van der. ‘Bayesian Analysis of Homogeneity Studies in the Production of Reference Materials’. Accreditation and Quality Assurance 22, no. 6 (2 November 2017): 307–19. https://doi.org/10.1007/s00769-017-1292-6.
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| Export citation | Export as RIS |
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| Collection | Canadian Reference Materials and Methods |
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| Record identifier | 5bff7c51-00c7-42ee-ba32-8b20d884b824 |
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| Record created | 2023-03-29 |
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| Record modified | 2024-04-04 |
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