| Alternative title | ACE2-1 : Matériau de référence protéique de l’enzyme de conversion de l’angiotensine 2 |
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| DOI | Resolve DOI: https://doi.org/10.4224/crm.2022.ace2-1 |
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| Author | Search for: Stocks, Bradley1ORCID identifier: https://orcid.org/0000-0002-7265-9344; Search for: Thibeault, Marie-Pier1; Search for: L'Abbé, Denis1; Search for: Stuible, Matthew1; Search for: Durocher, Yves1ORCID identifier: https://orcid.org/0000-0002-2268-4111; Search for: Melanson, Jeremy1ORCID identifier: https://orcid.org/0000-0002-2290-0101 |
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| Affiliation | - National Research Council of Canada
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| Format | 3D, Material |
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| Material type | Calibration solution |
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| Subject | Canadian reference material; ACE2-1; angiotensin-converting enzyme 2 protein |
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| Abstract | ACE2-1 is a protein reference material (RM) that serves as a source of angiotensin-converting enzyme 2 (ACE2) receptor for use in coronavirus spike protein binding assays, and for evaluating antibody-mediated inhibition of the receptor-spike interaction. The ACE2 protein in ACE2-1 is biotinylated to facilitate protein immobilization and/or affinity pull-down purifications. Reference values for the molar concentration, mass fraction, and mass concentration of human ACE2 protein in ACE2-1 have been established. Information values for the size heterogeneity of human ACE2 protein in ACE2-1 are provided. A unit of ACE2-1 contains 50 µL of human ACE2 in Dulbecco’s phosphate buffered saline. |
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| Analytes | | Analyte | Quantity | Value | Expanded uncertainty | Unit | Type |
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| angiotensin-converting enzyme 2 | molar concentration | 25.3 | 2.1 | µmol/L | reference | | angiotensin-converting enzyme 2 | mass fraction | 1.91 | 0.16 | mg/g | reference | | angiotensin-converting enzyme 2 | mass concentration | 1.93 | 0.16 | mg/mL | reference | | angiotensin-converting enzyme 2 | molar mass | 76107 | 1.0 | g/mol | reference | | angiotensin-converting enzyme 2 glycoprotein | molar concentration | 25.3 | 2.1 | µmol/L | reference | | angiotensin-converting enzyme 2 glycoprotein | mass fraction | 2.10 | 0.24 | mg/g | reference | | angiotensin-converting enzyme 2 glycoprotein | mass concentration | 2.13 | 0.24 | mg/mL | reference | | angiotensin-converting enzyme 2 glycoprotein | molar mass | 84000 | 7000 | g/mol | reference |
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| Publication date | 2022-02-01 |
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| Publisher | National Research Council of Canada |
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| Copyright statement | - © 2022
National Research Council of Canada
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| Access condition | |
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| References | - Guide to the expression of uncertainty in measurement (GUM) (1 January 1993). https://www.bipm.org/en/publications/guides.
- Veen, Adriaan M. H. van der. ‘Bayesian Analysis of Homogeneity Studies in the Production of Reference Materials’. Accreditation and Quality Assurance 22, no. 6 (2 November 2017): 307–19. https://doi.org/10.1007/s00769-017-1292-6.
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| Export citation | Export as RIS |
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| Collection | Canadian Reference Materials and Methods |
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| Record identifier | df43c24e-3213-4f26-94cd-dc273d988f6a |
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| Record created | 2022-02-21 |
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| Record modified | 2025-04-16 |
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